Fig 2: Correlation of sAC protein activity with sperm motility activity. (A1,A2) sAC protein activity was correlated with the proportion of forwardly motile spermatozoa. (B1,B2) Correlation between sAC protein activity and the proportion of X-chromosome-bearing sperm. (C,D) Statistics of the relative contents of p-PKAs and pY in dilutions of pH6.8 and pH7.4. Results are expressed as mean ± SEM, n = 3 experiments. Two-way ANOVA and Bonferroni’s multiple comparison test. (##) and (#) indicate significant differences between the two sets of data, respectively (p < 0.01) and (p < 0.05).

Fig 3: (A) Gel electrophoresis of the PCR amplification product of the sAC gene in dairy goat sperm. (B) Western blot of sAC from dairy goat sperm. (C) Changes in sAC protein activity when sperm were incubated in pH 7.4 dilution. (D,E) Relationship between sAC protein activity and pHi during sperm incubation. (F,G) Relationship between sAC protein activity and [Ca2+]i concentration during sperm incubation.